Educational Resources
The Colorado NWSS CoE is dedicated to enhancing the expertise surrounding wastewater surveillance systems. We have made it our mission to create comprehensive training programs that bolster knowledge in this field. Below, you can access all our current training materials.
Our commitment to excellence means that we are continuously working on developing new training resources. Be sure to visit our website frequently to stay updated on the latest offerings.
If you have specific topics in mind that you would like us to cover, please don't hesitate to contact us.
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Resources by Topic
Explore content such as informational videos, sample standard operating procedures and recorded training sessions.
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Public Health Communication Packages
Information designed to help public health departments communicate the importance of wastewater surveillance to their communities.
Document: Respiratory virus wastewater surveillance at the sewershed level (Flu/RSV)
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Overview of Wastewater Surveillance Systems
This section contains information for individuals contemplating the integration of a wastewater surveillance program but seeking guidance on how to incorporate it into their organization. Discover practical insights and strategies to effectively implement a wastewater surveillance initiative.
Video: An Overview of Wastewater Surveillance as a Public Health Tool- In this video, you will learn about wastewater surveillance and how public health agencies use wastewater data to serve their communities. The training includes an overview, a brief history, and the benefits and limitations of wastewater surveillance.
Partner Organization Document: Building COVID-19 Wastewater Surveillance with the National Wastewater Surveillance System (PDF)- Published by the Centers for Disease Control and Prevention.
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Levels of Wastewater Surveillance
Surveillance systems monitor populations at three different levels: Building-level, Sewershed-level and Community-Level. This section includes resources to help you determine which level is right for you.
Building-Level Surveillance
Document: Considerations when Evaluating a Building Wastewater System for Wastewater Surveillance-(PDF) This document provides important information to help you evaluate a particular building to determine if it is a good fit for building-level wastewater surveillance.
Document: Lessons Learned in Building-Level Wastewater Surveillance-(PDF) This document provides important information to help you evaluate a particular building to determine if it is a good fit for building-level wastewater surveillance.
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Collection Site Selection
Selecting the site (or sites) for collecting wastewater samples is an important decision, with numerous factors to weigh. In this section, you'll find materials designed to assist you with assessing the potential advantages and disadvantages of each site.
Video: Sample Site Selection- In this video, you will learn considerations for selecting and accessing your wastewater surveillance sampling site. A case study is used to provide concrete examples of how these considerations guide decisions throughout your project.
Document: Consideration for Site Selection Video Companion- Written summary of the video above.
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Sample Collection and Transportation
Information about the two most commonly used methods to collect wastewater samples- composite sampling and grab sampling.
Composite Sample Collection/Storage/Transport
A composite sample is collected from an autosampler that collects numerous small samples taken over regular intervals over a period of time, usually 24 hours.
Document: Example Standard Operating Procedure for Sample Collection/Storage/Transportation to Lab (Composite Samples)-This document describes procedures for safe transfer of wastewater samples from wastewater treatment plants to your state health department's laboratory for RNA extraction and analysis.
Grab Sample Collection/Storage/Transport
A grab sample is collected by lowering a specimen container into the sewer.
Video: Grab Sample Overview- This video provides an overview of the process of collecting grab samples for wastewater surveillance.
Document: Sample Standard Operating Procedure for Sample Collection/Storage/Transportation to Lab (Grab Samples)-This document outlines procedures for safe transfer of wastewater samples from wastewater treatment plants to your state health department's laboratory for RNA extraction and analysis.
- Partnerships with Wastewater Utilities and Public Health Agencies
Forming strong partnerships between wastewater utilities departments and public health agencies is one of the keys to wastewater surveillance program success. Resources in this section are designed to help you build and maintain these partnerships.
Document: Sample Memorandum of Understanding for Health Agencies and Wastewater Treatment Plants (PDF)- This document is an example of a Memorandum of Understanding between a state health department and a wastewater treatment plant. This document does not constitute legal advice. If you wish to implement an agreement regarding wastewater monitoring, you should consult with an attorney and obtain legal advice specific to your needs.
The purpose of this Memorandum of Understanding is to set forth the terms and conditions, scope of work, and responsibilities of the parties associated with their collaboration on wastewater surveillance. Download Word document
- Data Analysis and Sharing
Wastewater Surveillance Data Analysis and Dashboard Development Webinar Series
Audience: State public health staff involved in wastewater surveillance activities
Summary: The Colorado National Wastewater Surveillance System (NWSS) Center of Excellence (CoE) invites you to join our Wastewater Surveillance Data Analysis and Dashboard Development webinar series.
Presented By: Kirsten Weisbeck, MPH, Wastewater Data Analytics Supervisor, Colorado Department of Public Health and Environment
Part 1 (originally presented on July 12, 2023)
Focused on dashboard development and the following topics:
- Background on Colorado's approach to analyzing and displaying wastewater surveillance data
- The purpose of wastewater dashboards
- Key considerations when developing a dashboard
- Pros and cons of common dashboard platforms
- Resources for dashboard development
- A dashboard-focused Q & A
Part 2 (originally presented on January 17, 2024)
This webinar will focus on basic analytic methods and the interpretation of wastewater data and will include the following topics:
- Preparing data for analysis
- Identifying analytic objectives appropriate for each jurisdiction
- Reviewing normalization methods
- Analytic approaches for different sampling levels (sewershed, building-level, upstream)
- Analytic approaches for different targets (Flu, RSV, etc.)
- Routine analytics, including percentile calculation and trend analysis
- Advanced analytics, including aggregate measures and testing correlations
- Data communication
- Q & A
Links from this webinar:
- NWSS COVID Data Tracker (example of % change over time metric)
- NWSS Analytics Detailed Methodology (Reach out to the NWSS team if you need to request DCIPHER access: nwss@cdc.gov)
- Contour Training (You will need DCIPHER access)
- Houston Analysis and Reporting Guidelines
- BSTS Github
- Partnerships with Wastewater Utilities and Public Health Agencies
Recent Publications
Browse recent publications from our team of wastewater surveillance experts.
Read MoreFrequently Asked Questions
Read all of the questions we are typically asked about our Center of Excellence and wastewater surveillance.
Read MoreNWSS Communities of Practice
Communities of practice (CoP) are groups of professionals in various wastewater surveillance fields that generally meet on a monthly basis.
Health Department Community of Practice
Hosted by The Centers for Disease Control and Prevention (CDC) NWSS team
This CoP is a forum to allow state wastewater surveillance programs to receive updates from the CDC NWSS team, share ongoing work related to wastewater surveillance, and have direct access to leaders and peers in the field of wastewater surveillance. The NWSS health department CoP call is held on the fourth Thursday of each month from 1:00 - 2:00 pm ET. If you are interested in joining the NWSS CoP call, please email NWSS@cdc.gov.
Utility Community of Practice
Hosted by the Water Environment Federation
The NWSS Utility CoP is a collaborative for promoting review, synthesis, and sharing of lessons learned among wastewater utilities to accelerate implementation of wastewater surveillance and participation in the NWSS to support public health decision making. The Utility CoP is held on the third Thursday of each month from 3:00 - 4:00 pm ET. If you are interested in joining the Utility CoP call, please email nwbe@wef.org, or visit their website to learn more and fill out the request form.
Laboratory Community of Practice
Hosted by the Association of Public Health Laboratories (APHL)
The NWSS Laboratory CoP provides a forum for APHL member laboratories to discuss and share resources related to wastewater surveillance for SARS-CoV-2 and other analytes of interest. The Laboratory CoP call is scheduled for the second Monday of each month from 1:00-2:00 pm ET. If you are interested in joining the Laboratory CoP call, please email erin.morin@aphl.org.